dPC Technology
Biological samples are separated into fractions using parallel isoelectric focusing. Proteins and peptides are charged in an environment that is either above or below their isoelectric point, thus allowing them to migrate in an electric field.
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| Proteins/peptides at a pH higher than their pI (basic) will be negatively charged. Proteins/peptides at a pH below their pI (acidic) will be positively charged. Proteins/peptides at a pH equal to their pI will be uncharged. |
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| Proteins/peptides at a pH higher than their pI (basic) will be negatively charged. Proteins/peptides at a pH below their pI (acidic) will be positively charged. Proteins/peptides at a pH equal to their pI will be uncharged. |
The Digital ProteomeChip
By providing differences of 0.05 pH units between adjacent gel plugs, the digital ProteomeChip enables separation of proteins and peptides often found in the same fraction by other technologies. High-resolution fractionation facilitates study of charge isoforms, allows separation of high and low abundance species, and analysis of high molecular weight proteins.
- Separate proteins or peptides in 30–60 minutes
- Compatible with both simple and complex samples
- Identify the pI of specific proteins and peptides
- Eliminate variability associated with gel band cutting
- Achieve 105 depth of coverage
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| Digital Proteome Chip. |
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| Separation of alpha 2-macroglobulin and PKDP1 by pI in plasma, processed using the dPC platform. |
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