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Charge-based separation of peptides prior to LC-MS analysis has been hampered by long run times and the presence of components such as detergents and carrier ampholytes that interfere with MS (1). Cell Biosciences has developed a workflow using the digital ProteomeChip® (dPC®), that captures peptides in acrylamide gel plugs according to charge, with run times less than one hour (2,3) (figure 1). This workflow employs buffers and conditions that have been designed to be “MS-friendly,” eliminating the need for postseparation sample clean-up and dramatically reducing artifacts introduced by the separation processes. Cell Biosciences ProteomeChips are available in three pH ranges: 3.5-4.5, 4.2-6.2 and 6.0-8.0, providing an overall pH range of 3.5-8.0. |
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We present the development of novel nanoimmunoassays for the translational repressor proteins 4E-BP1 and 4E-BP2 using NanoPro technology. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase pathway regulate 4E-BP1 activity, making 4E-BP1 a focal point for these two important signaling pathways. 4E-BP2 regulation is poorly understood, partially due to the lack of specific anti-phospho 4E-BP2 antibodies. Our assay, developed on the Cell Biosciences NanoPro platform, enables detailed differential investigation of 4E-BP1 and 4E-BP2 phosphorylation and signal transduction. |
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