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Recent Literature

		NanoPro Premixes G2 (Separation Gradients)
		digital ProteomeChip (dPC) Fractionator Specifications
		Complex Peptide Mixture Fractionation via Parallel Isoelectric Focusing for Direct LC-MS/MS Analysis (ASMS 2010)         [show details]
		Charge-based separation of peptides prior to LC-MS analysis has been hampered by long run times and the presence of components such as detergents and carrier ampholytes that interfere with MS (1). Cell Biosciences has developed a workflow using the digital ProteomeChip® (dPC®), that captures peptides in acrylamide gel plugs according to charge, with run times less than one hour (2,3) (figure 1). This workflow employs buffers and conditions that have been designed to be “MS-friendly,” eliminating the need for postseparation sample clean-up and dramatically reducing artifacts introduced by the separation processes. Cell Biosciences ProteomeChips are available in three pH ranges: 3.5-4.5, 4.2-6.2 and 6.0-8.0, providing an overall pH range of 3.5-8.0.
		Application Brief 1025: FLAG Epitope Tag NanoPro Assay
		Application Brief 1026: HA Epitope Tag NanoPro Assay
		NanoPro Buffers
		NanoPro Premixes (Separation Gradients)
		NanoPro Master Kits
		NanoPro pI Standards MSDS
		Assay Development on the NanoPro Platform: 4E-BP1 and 4E-BP2 (SBS 2010)         [show details]
		We present the development of novel nanoimmunoassays for the translational repressor proteins 4E-BP1 and 4E-BP2 using NanoPro technology. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase pathway regulate 4E-BP1 activity, making 4E-BP1 a focal point for these two important signaling pathways. 4E-BP2 regulation is poorly understood, partially due to the lack of specific anti-phospho 4E-BP2 antibodies. Our assay, developed on the Cell Biosciences NanoPro platform, enables detailed differential investigation of 4E-BP1 and 4E-BP2 phosphorylation and signal transduction.